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KMID : 0545120120220040494
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 4 p.494 ~ p.500
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Enhancement of Anti-Inflammatory Activity of PEP-1-FK506 Binding Protein by Silk Fibroin Peptide
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Kim Dae-Won
Hwang Hyun-Sook
Kim Duk-Soo
Sheen Seung-Hoon
Heo Dong-Hwa
Hwang Gyo-Jun
Kang Suk-Hyung
Kweon Hae-Yong
Jo You-Young
Kang Seok-Woo
Lee Kwang-Gill
Park Jin-Seu
Eum Won-Sik
Cho Yong-Jun
Choi Soo-Young
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Abstract
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Silk fibroin (SF) peptide has been traditionally used as a treatment for flatulence, spasms, and phlegm. In this study, we examined whether SF peptide enhanced the antiinflammatory effect of PEP-1-FK506 binding protein (PEP-1-FK506BP) through comparing the anti-inflammatory activities of SF peptide and/or PEP-1-FK506BP. In the presence or absence of SF peptide, transduction levels of PEP-1-FK506BP into HaCaT cells and mice skin and anti-inflammatory activities of PEP-1-FK506BP were identified by Western blot and histological analyses. SF peptide alone effectively reduced both mice ear edema and the elevated levels of cyclooxygenase-2, interleukin-6 and -1β, and tumor necrosis factor-α, showing similar anti-inflammatory effect to that of PEP-1-FK506BP. Furthermore, co-treatment with SF peptide and PEP-1- FK506BP exhibited more enhanced anti-inflammatory effects than the samples treated with SF peptides or PEP- 1-FK506BP alone, suggesting the possibility that SF peptide and PEP-1-FK506BP might interact with each other. Moreover, the transduction data demonstrated that SF peptide did not affect the transduction of PEP-1- FK506BP into HaCaT cells and mice skin, indicating that the improvement of anti-inflammatory effect of PEP-1- FK506BP was not caused by enhanced transduction of PEP-1-FK506BP. Thus, these results suggest the possibility that co-treatment with SF peptide and PEP-1-FK506BP may be exploited as a useful therapy for various inflammationrelated diseases.
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KEYWORD
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Inflammation, PEP-1-FK506BP, Protein transduction, Reactive oxygen species, Silk fibroin peptide, TPA
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